RPAD (RNase R treatment, polyadenylation, and poly(A)+ RNA depletion) method to isolate highly pure circular RNA
نویسندگان
چکیده
منابع مشابه
High-purity circular RNA isolation method (RPAD) reveals vast collection of intronic circRNAs
High-throughput RNA sequencing methods coupled with specialized bioinformatic analyses have recently uncovered tens of thousands of unique circular (circ)RNAs, but their complete sequences, genes of origin and functions are largely unknown. Given that circRNAs lack free ends and are thus relatively stable, their association with microRNAs (miRNAs) and RNA-binding proteins (RBPs) can influence g...
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ژورنال
عنوان ژورنال: Methods
سال: 2019
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2018.10.022